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PROCESS DESIGN - Butanol Production from Biomass

PROCESS DESIGN - Butanol Production from Biomass

Saccharification occurs in a CSTR reactor with 349 kilograms of water. With an estimate of 25 g of enzyme needed for 1 kg of cellulosic feedstock, we determined a need for 400.4 g of enzyme necessary. It has been shown to be feasible to use clostridium to produce its own saccharifying agents. This hasn't been well studied, however, and thus we .

Kinetic modeling of countercurrent saccharification .

Kinetic modeling of countercurrent saccharification .

Jul 11, 2019 · Reactor volume: This ensures the same capital cost. Using the method in "Reactor volume calculation" section, the LRT in countercurrent saccharification is adjusted to reach the same reactor volume as batch saccharification. Some industrial reactors, such as percolation reactors, allow for high solid concentrations, which reduces capital costs.

Enzymatic saccharification of solid residue of olive mill .

Enzymatic saccharification of solid residue of olive mill .

The overall enzyme activity loss after a 5 h diafiltration period was 28%, however about half this loss appeared to be due to enzyme denaturation inside the reactor. During saccharification trials .

(PDF) Ethanol Production from High Solid Loading of Rice .

(PDF) Ethanol Production from High Solid Loading of Rice .

Ethanol Production from High Solid Loading of Rice Straw by Simultaneous Saccharification and Fermentation in a Non-Conventional Reactor Article (PDF Available) in Energies 13(8):1-17 · April .

Modeling fixed and fluidized reactors for cassava starch .

Modeling fixed and fluidized reactors for cassava starch .

Cassava starch saccharification in fixed-and fluidized-bed reactors using immobilized enzyme was modeled in a previous paper using a simple model in which all dextrins were grouped in a single substrate. In that case, although good fit of the model to experimental data was obtained, physical .

Saccharification of Cellulose Using a Hot-Compressed Water .

Saccharification of Cellulose Using a Hot-Compressed Water .

Cellulose was hydrolyzed with hot-compressed water (HCW; ∼310 °C, 9.8 MPa) using an HCW-flow reactor. HCW was continuously delivered into a reactor charged with cellulose. The effluent from the reactor was cooled and separated into a water-soluble fraction (WS) and a water-insoluble fraction which deposited after cooling (DP). Cellulose started to decompose into WS and DP when HCW was .

Hydrolysis - Wikipedia

Hydrolysis - Wikipedia

Hydrolysis (/ h aɪ ˈ d r ɒ l ɪ s ɪ s /; from Ancient Greek hydro-, meaning 'water', and lysis, meaning 'to unbind') is any chemical reaction in which a molecule of water ruptures one or more chemical bonds.The term is used broadly for substitution, elimination, and solvation reactions in which water is the nucleophile.. Biological hydrolysis is the cleavage of biomolecules where a water .

Nonisothermal simultaneous saccharification and .

Nonisothermal simultaneous saccharification and .

The NSSF is devised so that saccharification and fermentation occur simultaneously, yet in two separate reactors that are maintained at different temperatures. Lignocellulosic biomass is retained inside a column reactor and hydrolyzed at the optimum .

A model for continuous enzymatic saccharification of .

A model for continuous enzymatic saccharification of .

David Fox, Noel W. Dunn, Peter P. Gray and Warwick L. Marsden, Saccharification of bagasse using a counter‐current plug‐flow reactor, Journal of Chemical Technology and Biotechnology. Biotechnology, 33, 2, (114-118), (2008).

Deconstruction: Saccharification - ABPDU

Deconstruction: Saccharification - ABPDU

Saccharification, the process to depolymerize cellulose and hemicellulose into fermentable sugars, is a considerable cost component in the biochemical conversion of biomass and feedstock to bioproducts. In developing a high-yielding saccharification process, we take into consideration a combination of several factors: Biomass composition

Saccharification of cassava starch in an ultrafiltration .

Saccharification of cassava starch in an ultrafiltration .

Saccharification of cassava starch in an ultrafiltration reactor D. Darnoko*, M. Cheryant and W. E. Artz University of Illinois, 382D A.E.S. Building, 1304 West Pennsylvania Avenue, Urbana, IL 61801 (Received 26 November 1987; revised 3 May 1988) Extruded cassava starch was continuously hydrolysed with glucoamylase in an ultrafiltration (UF) reactor.

Fungal biomass and ethanol from lignocelluloses using .

Fungal biomass and ethanol from lignocelluloses using .

simultaneous saccharification, filtration, and fermentation (SSFF) was developed by Ishola et al. (2013). This new concept consists of a membrane unit (cross-flow membrane) connecting a hydrolysis reactor to a fermentation reactor. The enzyme-slurry mixture from the hydrolysis reactor is filtered and

Saccharification of potato starch in an ultrafiltration .

Saccharification of potato starch in an ultrafiltration .

A new saccharification assay has been devised, in which a continuously buffer-swept membrane reactor is used to remove the solubilized saccharification products, thus allowing high extents of .

One-pot NaOH/urea pretreatment and saccharification of .

One-pot NaOH/urea pretreatment and saccharification of .

This one-pot production of fermentable sugars was achieved via a combination process, including pretreatment with low-temperature NaOH/urea solution, pH adjustment, and enzymatic saccharification in a single reactor. This one-pot process liberated 86.3% of glucose and 71.3% of xylose in 24 h at an enzyme loading of 10 FPU/g and solid loading of 5%.

Enzymatic saccharification of solid residue of olive mill .

Enzymatic saccharification of solid residue of olive mill .

The overall enzyme activity loss after a 5 h diafiltration period was 28%, however about half this loss appeared to be due to enzyme denaturation inside the reactor. During saccharification trials .

Saccharification method - Gulf Research & Development Company

Saccharification method - Gulf Research & Development Company

Oct 11, 1983 · As discussed herein above, it is possible to operate the simultaneous saccharification and fermentation reactor disclosed herein in a "plug flow" mode; and operation in such a mode with countercurrent flow of fresh cellulosic feedstock and effluent solution, as shown schematically in Fig. 5, can be beneficial and desirable.

Nonisothermal simultaneous saccharification and .

Nonisothermal simultaneous saccharification and .

The NSSF is devised so that saccharification and fermentation occur simultaneously, yet in two separate reactors that are maintained at different temperatures. Lignocellulosic biomass is retained inside a column reactor and hydrolyzed at the optimum .

Laboratory-scale method for enzymatic saccharification of .

Laboratory-scale method for enzymatic saccharification of .

The roller bottle reactor (RBR) system was well mixed in every instance of continuous rolling. Therefore, our results showed that a high-solids enzymatic saccharification method requiring the least user intervention (that is the RBR) would be the most efficient system for this work.

Bioreactors for lignocellulose conversion into fermentable .

Bioreactors for lignocellulose conversion into fermentable .

Nov 16, 2015 · The reactor was rotated at 5 rpm for 1 min only at the beginning of the process and every 24 h. After 72 h, 24.6 g L −1 of ethanol concentration (79.0 % ethanol yield) were obtained. A particular reactor system has been set up by Ishola et al. using simultaneous saccharification, filtration and fermentation (SSFF) process. This system .

Demonstration‐scale enzymatic saccharification of sulfite .

Demonstration‐scale enzymatic saccharification of sulfite .

The saccharification reactions were started in fed‐batch using Reactor 1. Substrate with approximately 30% (w/w) DM was gradually fed into the reactor containing enzymes and SSL, to a targeted final working weight of approximately 4000 kg, substrate loading of 12% (w/w) DM pulp and enzyme loading of 4% (w liquid/w DM of substrate).

A Scraped Surface Bioreactor for Enzymatic .

A Scraped Surface Bioreactor for Enzymatic .

Nov 14, 2008 · An 8 L scraped surface bioreactor (SSBR) is employed here for enzymatic saccharification experiments to handle high solids loading and as a means for scale-up from laboratory-scale shake flasks. The scraping action of the blades keeps the reactor surface clear, which improves the heat-transfer characteristics.

Intensification of Bioethanol Production by Simultaneous .

Intensification of Bioethanol Production by Simultaneous .

Intensification of Bioethanol Production by Simultaneous Saccharification and Fermentation (SSF) in an Oscillatory Baffled Reactor (OBR) Joseph Ikwebe. 1* and Adam P Harvey. 1. 1. Newcastle University, Newcastle upon Tyne, United Kingdom *Corresponding author. Tel. +441912227169, Email: [email protected] . Abstract:

Enzymatic saccharification of pretreated corn stover in a .

Enzymatic saccharification of pretreated corn stover in a .

Dec 18, 2010 · Enzymatic hydrolysis of corn stover was performed in an integrated membrane bioreactor (MBR) incorporating a 10 kDa flat sheet polysulfone membrane to increase cellulose conversion and to reduce enzyme dosage. Several pretreatment methods and semi-continuous MBR were examined to investigate their effect on the glucose yield and enzyme utilization efficiency.

Enzymatic membrane reactor for full saccharification of .

Enzymatic membrane reactor for full saccharification of .

Membrane reactor for enzymatic saccharification of RC under semi-continuous operation mode As can be seen in Fig. 1, the reactor system consisted of a stirred tank (100-mL total capacity) connected through a peristaltic pump with an ultrafiltration Vivaflow-50 unit based on PES membranes (50 cm 2 overall surface, 10 or 5 kDa cut-off).

(PDF) Enzymatic saccharification of cellulose: A study of .

(PDF) Enzymatic saccharification of cellulose: A study of .

Simultaneous saccharification and fermentation (SSF) of cassava in an oscillatory baffled reactor (OBR) and in a stirred tank reactor (STR) was studied at various agitation regimes.

Control of High-Solids Saccharification using a Model .

Control of High-Solids Saccharification using a Model .

level during saccharification to overcome rheological limitations in the reactor as in the work of Ballesteros et al. (2002) for SSF by Kluyveromyces marxianus. In this work, the insoluble solids were increased from 10% to 20%, which the reactor would have been incapable of handling initially, as well as reducing the effects of inhibitors in the

Performance targets defined by retro-techno-economic .

Performance targets defined by retro-techno-economic .

Apr 30, 2020 · Nevertheless, the results showed that when the overall biorefinery was considered, an increase in the bagasse loading in the saccharification reactor had a .

High-Solids Enzymatic Saccharification Screening Method .

High-Solids Enzymatic Saccharification Screening Method .

May 01, 2009 · Shake flasks do not provide adequate mixing at high solids conditions. In this work, a roller bottle reactor was identified as a small-scale high-solids saccharification reaction vessel, and a method was developed for use in screening both pretreated biomass and enzyme systems at process-relevant conditions.

Enzymatic saccharification of cellulose: a study of mixing .

Enzymatic saccharification of cellulose: a study of mixing .

Cellulose saccharification has been shown to be a function of agitation. Here, the effect of agitation by oscillatory mixing in an oscillatory baffled reactor (OBR) has been assessed and contrasted with a stirred tank reactor (STR). After 168 h of saccharification at 200 Wm−3, 91% conversion of the cellulose (∼25 g/L glucose) was observed in the OBR, as against 74% conversion (∼21 g/L .

Fungal biomass and ethanol from lignocelluloses using .

Fungal biomass and ethanol from lignocelluloses using .

simultaneous saccharification, filtration, and fermentation (SSFF) was developed by Ishola et al. (2013). This new concept consists of a membrane unit (cross-flow membrane) connecting a hydrolysis reactor to a fermentation reactor. The enzyme-slurry mixture from the hydrolysis reactor is filtered and